The endophytic fungus, rich in potent bioactive compounds, exhibited notable antibacterial efficacy against pathogenic microorganisms. Our study delved into the efficacy of A. fumigatus, using in-vitro techniques to assess the bioactive potential (antibacterial and anticancer activity) of ethyl acetate extract from fermented broth, containing secondary metabolites. The crude extract demonstrated significant antibacterial activity against Multi-Drug Resistant (MDR) strains S. aureus and K. pneumoniae, and promising anti-cancer activity against HeLa cells. Chemical analysis of fermented extract via GC-MS unveiled a diverse array of constituents. In-silico analysis highlighted two compounds i.e. 1,3,6,9 b-tetraazaphenalene-4-carbonitrile and 4- isothiazolecarboxylic acid, 3,5-bis(methylthio)-, methyl ester, that are actively participating in biological activities, corroborated by in-vitro experiments. These findings underscore A. fumigatus' potential as a natural source of antibacterial and anticancer agents, with favorable pharmacokinetics and docking profiles, promising as drug candidates with minimal toxicity. Further research should explore their potential therapeutic applications through pre-clinical in-vivo studies.
Bhowmick et al. (Wed,) studied this question.