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September 10, 2025Open Access

Molecular detection of multidrug resistance Klebsiella species isolates from urinary tract patients in Thi-Qar province

Key Points

About 45.67% of isolated Klebsiella pneumoniae show resistance to antibiotics, indicating a significant challenge in treatment.
PCR amplification for 16S rRNA helped identify the isolates, revealing the presence of virulence genes in the bacteria.
Men exhibited a higher prevalence of urinary tract infections caused by Klebsiella pneumoniae, with infection rates at 77.97%.
The study emphasizes the need for further research on the epidemiology of Klebsiella pneumoniae across Iraq to enhance treatment strategies.

Abstract

The bacterium causing urinary tract infections (UTIs), Klebsiella pneumoniae is a major globally. It is a gram-negative bacterial genus, a member of the Enterobacteriaceae family. It is a rod-shaped, non-motile, non-spore-forming with a prominent capsule made of polysaccharides. This study designed to isolate and identify Klebsiella species from urinary tract patients in Thi-Qar province. One hundred specimens were collected from UTIs-infected patients, ageing (11) to (76) years, .in three hospitals (Al-Hussein Teaching Hospital, Al-Nasiriya Teaching Hospital, and Bint Al-Huda Hospital) in Thi-Qar province between November 2023 and March 2024.The isolates were identified and their features concerning antibiotic resistance were investigated using the VITEK II system.Then examining the isolated bacterial proteins started with the polymerase chain reaction (PCR) assay identifying 16S rRNA . Results showed that rmpA, AcrAB, and fimH1 were the virulence genes. On the culture medium, the most commonly observed gram-positive bacteria were 25 (30.49%), E. coli 19 (23.17%), and K. pneumoniae 33 (40.24%).Using the VITEK II system revealed 33 samples including Klebsiella spp. bacterial presence. Men had the highest rate (77.97%) while the women rate (59.02%) regarding bacterial infections. Examining 70 drugs against K. pneumoniae, the study found that 45.67% of isolated bacteria were resistant to antibiotics, 9.91% were intermediate, and 44.42% of the bacteria were sensitive to antibiotics. Comprising 100% Ampicillin, 93.94% Amoxicillin, and 63.64% Ceftriaxone. The study revealed that successful treatments for K. pneumoniae are Cefpirome and Faropenem. The study finds isolates of 22 Klebsiella pneumoniae. PCR results of Gram-negative bacterial amplification helped to identify the isolates by means of the 16S rRNA amplification. According to the research, isolates of Klebsiella pneumoniae show great potential as targets for antibiotics and other medications. Therefore, the authors request a future study to determine the epidemiology of Klebsiella pneumoniae in all Iraqi provinces.

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Cite This Study

Salih et al. (Fri,) studied this question.

synapsesocial.com/papers/68c199ee9b7b07f3a061bce2 https://doi.org/https://doi.org/10.58564/jmob.61

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