Abstract: The development of RP HPLC methods is not without difficulties, despite its many advantages. These difficulties include problems with column stability, sample preparation, and optimization. This article describes new techniques that can be used with the RP-HPLC process to design and produce novel pharmaceuticals. Additionally covered are instances of more current developments in hyphenated methods like RP-HPLC. We intend to stimulate ongoing innovation and advancement in the developing field of pharmacy by examining the most recent developments, applications, and difficulties in the creation of RP-HPLC methods. Keywords: RP-HPLC, LC-MS and API (Active Pharmaceutical Ingredient)
Chandra et al. (Thu,) studied this question.
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