The present study is aimed with an objective to produce bio ethanol from agrowaste. Paddy straw bio mass is collected from agricultural land and utilized as feed stock for ethanol production after subjecting them to acid, alkali and enzymatic pre treatments. Two bacterial strains (R2 and R3) and one yeast strain (S6) were isolated from soil samples collected from paddy field dumped with straw and from sugarcane bagasse dumped soil. In addition to this, cellulose degrading bacteria strain (C1) is isolated to carry out the enzymatic pre treatment. Based on the fermentation efficiency tested, S6, R2 and R3 strains were inoculated in eight different pretreated biomass filtrate medium for fermentation process. Ethanol concentration were quantified at a period of five days interval up to twenty days. Ethanol concentration increased from 5th day to 20th day and 5th day to 15th day in yeast and bacteria inoculated medium. After recovery through distillation, yeast strain S6 inoculated medium is reported with more concentration of ethanol in alkali pre treated bio mass filtrate medium. There is no significant difference between alkali pre treated biomass and combined alkali & enzyme pre treated bio mass inoculated with yeast strain at 0.001 level of significance. The overall product extracted is in the range of 5-12%. Ethanol extracted from yeast S6 is reported to have disinfectant effect against bacteria. FTIR spectrum analysis reported the presence of similar peaks to that of standard ethanol
Nalini et al. (Tue,) studied this question.
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