The gut microbiome plays a critical role in host physiology and adaptation, shaped by both intrinsic host factors and extrinsic environmental conditions. In this study, we investigated the influence of habitat type and geographical isolation on gut microbial communities in habitat-isolated populations of the euryhaline cichlid Etroplus suratensis, which inhabit freshwater and brackish water environments. Using 16S rRNA gene amplicon sequencing, we compared microbial assemblages in fish guts and their corresponding habitats to assess patterns of community divergence. Alpha and beta diversity analyses revealed significant differences in microbial composition between gut and water samples, with limited overlap, particularly in brackish water, indicating strong host-mediated filtering of environmental microbiota. Notably, brackish and freshwater habitats harbored 2244 and 3136 unique water-associated taxa, respectively, while only 36 and 426 taxa were shared between water and gut in each habitat. Despite habitat divergence, 59 microbial taxa were consistently shared across gut samples from both populations, indicating the existence of a conserved core microbiome that likely fulfills essential functional roles. These findings support the notion that the fish gut serves as a selective ecological niche, enabling the persistence of functionally relevant microbes while restricting the entry of environmental transients. Moreover, the observed divergence in gut microbiota across habitats, coupled with a shared core, highlights the interplay between local adaptation and conserved host–microbe associations, with potential implications for understanding microbial contributions to vertebrate ecological diversification and allopatric speciation.
José et al. (Tue,) studied this question.