Key points are not available for this paper at this time.
The endomembrane system of cereal seed endosperm is a highly plastic and dynamic system reflecting the high degree of specialization of this tissue. It is capable of coping with high levels of protein synthesis and undergoes rapid changes to accommodate these storage proteins in newly formed storage organelles such as ER-derived protein bodies (PBs) or protein storage vacuoles (PSVs). The study of endomembrane morphology in cereal endosperm is challenging due to the amount of starch that cereal seeds accumulate and the progressive desiccation of the tissue. Here we present a comprehensive study of the endomembrane system of developing barley endosperm cells, complemented by CLEM imaging. The use of genetically fused fluorescent protein tags in combination with the high resolution of electron microscopy brings ultrastructural research to a new level and can be used to generate novel insights in cell biology in general and in cereal seed research in particular.
Huber et al. (Fri,) studied this question.