Bin2cell reconstructs cells from high resolution Visium HD data

Abstract Summary Visium HD by 10X Genomics is the first commercially available platform capable of capturing full scale transcriptomic data paired with a reference morphology image from archived FFPE blocks at sub-cellular resolution. However, aggregation of capture regions to single cells poses challenges. Bin2cell reconstructs cells from the highest resolution data (2 μ m bins) by leveraging morphology image segmentation and gene expression information. It is compatible with established Python single cell and spatial transcriptomics software, and operates efficiently in a matter of minutes without requiring a GPU. We demonstrate improvements in downstream analysis when using the reconstructed cells over default 8 μ m bins on mouse brain and human colorectal cancer data. Availability and Implementation Bin2cell is available at https://github.com/Teichlab/bin2cell , along with documentation and usage examples, and can be installed from pip. Probe design functionality is available at https://github.com/Teichlab/gene2probe Contact ny1@sanger.ac.uk , st9@sanger.ac.uk Supplementary information Supplementary data are available online.