Aktivitas Antibakteri Eco Enzyme Terhadap Bakteri Yang Diisolasi Dari Rumput Laut Terinfeksi Ice-Ice

Eco enzyme is the result of fruit peel fermentation mixed with brown sugar water. Eco enzyme contains acetic acid that can inhibit the growth of bacteria, including bacteria isolated from seaweed infected with ice-ice disease. The purpose of this study was to analyze the antibacterial activity of eco enzyme to inhibit bacteria isolated from seaweed infected with ice-ice. This research was conducted in July-November 2023. The inhibition test was conducted at the BPBL Ambon Testing Laboratory, while the phytochemical test was conducted at the Herbal Materia Medica Laboratory in Batu, Malang. Eco enzyme was made at the Aquatic Resource Management Laboratory, Faculty of Fisheries and Marine Science, Pattimura University for three months. The method for antibacterial test of eco enzyme used disc diffusion method. There are four types of bacteria that have been isolated from ice-ice infected seaweed, namely Vibrio alginolyticus, Vibrio fluvalis, Vibrio cholerae, and Aeromonas cavie. There are four concentrations of eco enzyme used to inhibit the growth of isolated bacteria, namely 25%, 50%, 75% and 100%. Phytochemical test results showed that the eco enzyme solution contained phenol of 319.0921 mg GA/g, total flavonoids of 133.5000 mg Q/g, total tannins of 224.6916 mg ET/g, and IC50 value on DPPH (1,1-diphenyl-2-picrylhydrazil) method of 290.9558 ppm. Based on the results of the inhibition test, the greater the concentration of eco enzyme, the greater the inhibition zone produced. Inhibition zone with moderate category was found in bacteria V. alginolticus and A. cavie with 100% concentration, while others were included in the weak category. ABSTRAK Eco enzyme merupakan hasil fermentasi kulit buah dicampur dengan gula merah air. Eco enzyme mengandung asam asetat yang dapat menghambat pertumbuhan bakteri, antara lain bakteri yang diisolasi dari rumput laut yang terinfeksi penyakit ice-ice. Tujuan dari penelitian ini yaitu menganalisis aktivitas antibakteri eco enzyme untuk menghambat bakteri yang diisolasi dari rumput laut terinfeksi ice-ice. Penelitian ini dilakukan pada Juli-November 2023. Uji hambat dilakukan di Laboratorium Penguji BPBL Ambon, sedangkan untuk uji fitokimia dilakukan di Laboratorium Herbal Materia Medica Batu, Malang. Eco enzyme dibuat di Laboratorium MSP FPIK UNPATTI selama tiga bulan. Metode untuk uji antibakteri eco enzyme menggunakan metode difusi cakram. Terdapat empat jenis bakteri yang telah diisolasi dari rumput laut terinfeksi ice-ice yaitu Vibrio alginolyticus, Vibrio fluvalis, Vibrio cholerae, dan Aeromonas cavie. Terdapat empat konsentrasi eco enzyme yang digunakan untuk menghambat pertumbuhan bakteri isolasi yaitu 25%, 50%, 75% dan 100%. Hasil uji fitokimia menunjukan bahwa larutan eco enzyme mengandung Fenol sebesar 319,0921 mg GA/g, total Flavonoid sebesar 133,5000 mg Q/g, total Tanin sebesar 224,6916 mg ET/g, dan nilai IC50 pada metode DPPH (1,1-diphenil-2-picrylhydrazil) sebesar 290,9558 ppm. Berdasarkan hasil uji hambat diperoleh semakin besar konsentrasi eco enzyme maka semakin besar pula zona hambat yang dihasilkan. Zona hambat dengan kategori sedang ditemukan pada bakteri V. alginolticus dan A. cavie dengan konsentrasi 100%, sedangkan lainnya termasuk dalam kategori lemah. Kata Kunci: Eco enzyme, antibakteri, fitokimia, rumput laut, ice-ice