FIGURE 5 from Humanized Patient-derived Xenograft Models of Disseminated Ovarian Cancer Recapitulate Key Aspects of the Tumor Immune Environment within the Peritoneal Cavity

Detection of Human TILs in huNBSGW PDX tumor sections. A, Tumor sections from huPDX were labeled for human T-cell markers CD3, CD4, and CD8. Shown are scanned images of labeled sections at 20× magnification. Representative images are shown for huPDX3 and huPDX18. For huPDX9, the image shown is a rare group of TILs in a mostly negative section to show that the rare T cells were being labeled. Scale bar, 100 μm. B, Quantification of the number of TILs using HALO software. Values are reported as no. of positive cells/100,000 cells. The box plots indicate the mean, minimum, and maximum. Black dots indicate values for each tumor analyzed (n = 2–3).