Growth Assessment of Spirulina platensis under Oxidative Stress

Arthrospira platensis, the spiral, multicellular, filamentous, non-heterocystus, non-nitrogen-fixing photosynthetic cyanobacteria that make up spirulina, is an entirely biologically derived organism. Spirulina can tolerate varied fluctuations in H2O2 and requires an abundance of minerals. The current work investigates the possibility of enhanced growth and cultivation under stressful circumstances, like variations in concentration of H2O2.Different concentrations of H2O2 under which S. platensis untreated, 2, 4, 6, 8, and 10 mM have been grown for 20 days. S. platensis cells, grown under photoperiod of 12 hours light/dark provided by fluorescent lamps with the light intensity of 140 µmol photons m-2s-1) in optimized Zarrouk's medium under controlled conditions. The dry biomass of algal (expressed as mgDW/ml) was moderately reduced under H2O2 stress with the exposure time and different H2O2 concentration. The present study observed that the biomass stimulated at lower oxidative stress concentration which ranges from 2mM (105mg DW/250ml culture) to 10mM(20mg DW/ml) but decreased at higher concentration as algal cells undergo disruption and results into inhibition of many cellular processes at high concentration.