Thiram Effects on HeLa TI Cells

firstₚage Download PDF settings Order Article Reprints Font Type: Arial Georgia Verdana Font Size: Aa Aa Aa Line Spacing:    Column Width:    Background: Open AccessAbstract Thiram Effects on HeLa TI Cells † by Varvara MaksimovaVarvara Maksimova SciProfiles Scilit Preprints. org Google Scholar 1, *, Anzhelika BukinaAnzhelika Bukina SciProfiles Scilit Preprints. org Google Scholar 1, 2, Guzel KhayrievaGuzel Khayrieva SciProfiles Scilit Preprints. org Google Scholar 3, Valeriia PopovaValeriia Popova SciProfiles Scilit Preprints. org Google Scholar 1, Marianna YakubovskayaMarianna Yakubovskaya SciProfiles Scilit Preprints. org Google Scholar 1 and Kirill KirsanovKirill Kirsanov SciProfiles Scilit Preprints. org Google Scholar 1 1 Department of Chemical Carcinogenesis, N. N. Blokhin National Medical Research Center of Oncology, 115478 Moscow, Russia 2 Department of Biotechnology and Industrial Pharmacy, Lomonosov Institute of Fine Chemical Technologies, MIREA—Russian Technological University, 86 Vernadsky Ave. , 119571 Moscow, Russia 3 Institute of Clinical Medicine, I. M. Sechenov First Moscow State Medical University, 8-2 Trubetskaya Str. , 119991 Moscow, Russia * Author to whom correspondence should be addressed. † Presented at the 1st International Electronic Conference on Toxics, 20–22 March 2024; Available online: https: //sciforum. net/event/IECTO2024. Proceedings 2024, 102 (1), 35; https: //doi. org/10. 3390/proceedings2024102035 Published: 3 April 2024 Download keyboardₐrrowdown Download PDF Download PDF with Cover Download XML Download Epub Download Supplementary Material Versions Notes Keywords: thiram; pesticide; HeLa TI cells; DNA damage; colony formation; proliferation; epigenetic activity 1. IntroductionDithiocarbamate pesticides possess a diverse array of molecular mechanisms, making them multifunctional substances. Among the commonly used dithiocarbamates, there is the fungicide thiram, employed for safeguarding plants and seeds against fungal diseases. The limited solubility of thiram in water facilitates its accumulation in the soil, thereby raising concerns about its potential impact on human health. Despite the widespread use of thiram, there is still a paucity of knowledge regarding its toxic effects on humans. This study aimed to assess the genotoxic effects of thiram, its influence on cell colony formation, and its effect on the expression profile of genes associated with proliferation and repair. Additionally, we conducted an analysis of thiram's integral epigenetic activity under different time exposures. 2. Materials and MethodsHeLa TI cells were used in this study. DNA damage was assessed using the DNA comet assay, while the clonogenic assay was utilized to evaluate the colony-forming ability of cells after thiram treatment. Gene expression levels were measured using real-time PCR. The epigenetic activity of thiram was assessed by quantifying the reactivation level of the epigenetically silenced GFP gene utilizing flow cytometry. 3. ResultsUsing HeLa TI cells, we observed that thiram significantly increased tail moment in the DNA comet assay and caused a significant decrease in cell survival as determined by the clonogenic assay. Additionally, thiram downregulated the gene expression levels of DDB2, ERCC5, LIG4, and WEE1, while upregulating the expression levels of MCM2, DDIT3, ERCC3, JUN1, and ESR1. The analysis of epigenetic activity revealed that thiram induced a statistically significant increase in the GFP+ cells amount after exposures of 24, 72, and 96 h. 4. ConclusionsThus, thiram exhibits genotoxic and cytotoxic effects, influences the expression of proliferation and repair genes, and induces dose- and time-dependent epigenetic modifications in HeLa TI cells. Supplementary MaterialsThe following are available online at https: //www. mdpi. com/article/10. 3390/proceedings2024102035/s1. Author ContributionsConceptualization, V. M. , M. Y. and K. K. ; methodology, V. M. ; formal analysis, V. M. , A. B. , V. P. and G. K. ; investigation, V. M. , A. B. , V. P. and G. K. ; resources, K. K. ; data curation, V. M. ; writing—original draft preparation, V. M. ; writing—review and editing, M. Y. and K. K. ; visualization, V. M. ; supervision, K. K. ; project administration, V. M. ; funding acquisition, V. M. All authors have read and agreed to the published version of the manuscript. FundingThis research was funded by Russian Scientific Foundation, grant number 23-25-00541. Institutional Review Board StatementNot applicable. Informed Consent StatementNot applicable. Data Availability StatementData available on request. Conflicts of InterestThe authors declare no conflict of interest. Disclaimer/Publisher's Note: The statements, opinions and data contained in all publications are solely those of the individual author (s) and contributor (s) and not of MDPI and/or the editor (s). MDPI and/or the editor (s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https: //creativecommons. org/licenses/by/4. 0/). Share and Cite MDPI and ACS Style Maksimova, V. ; Bukina, A. ; Khayrieva, G. ; Popova, V. ; Yakubovskaya, M. ; Kirsanov, K. Thiram Effects on HeLa TI Cells. Proceedings 2024, 102, 35. https: //doi. org/10. 3390/proceedings2024102035 AMA Style Maksimova V, Bukina A, Khayrieva G, Popova V, Yakubovskaya M, Kirsanov K. Thiram Effects on HeLa TI Cells. Proceedings. 2024; 102 (1): 35. https: //doi. org/10. 3390/proceedings2024102035 Chicago/Turabian Style Maksimova, Varvara, Anzhelika Bukina, Guzel Khayrieva, Valeriia Popova, Marianna Yakubovskaya, and Kirill Kirsanov. 2024. "Thiram Effects on HeLa TI Cells" Proceedings 102, no. 1: 35. https: //doi. org/10. 3390/proceedings2024102035 Article Metrics No No Article Access Statistics Multiple requests from the same IP address are counted as one view.