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Abstract Extracellular matrix (ECM) is an intricate structure providing the microenvironment niche that influences stem cell differentiation. This study aimed to investigate the efficacy of decellularized ECM derived from human dental pulp stem cells (dECMDPSCs) and gingival-derived mesenchymal stem cells (dECMGSCs) as an inductive scaffold for osteogenic differentiation of GSCs. The proteomic analysis demonstrated that common and signature matrisome proteins from dECMDPSCs and dECMGSCs were related to osteogenesis/osteogenic differentiation. RNA sequencing data from GSCs reseeded on dECMDPSCs revealed that dECMDPSCs upregulated genes related to the Hippo and Wnt signaling pathways in GSCs. In the inhibitor experiments, results revealed that dECMDPSCs superiorly promoted GSCs osteogenic differentiation, mainly mediated through Hippo and Wnt signaling. The present study emphasizes the promising translational application of dECMDPSCs as a bio-scaffold rich in favorable regenerative microenvironment for tissue engineering.
Kornsuthisopon et al. (Thu,) studied this question.
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