Coagulation dysfunction, a common hematologic disorder with unclear pathogenesis, is influenced by environmental factors. Sodium dehydroacetate (SDA), a widely used preservative with high environmental mobility and persistence, has become an emerging organic contaminant and is increasingly recognized for its potential to disrupt immune homeostasis and induce coagulation abnormalities, yet its specific mechanisms remain poorly understood. In this study, we employed an integrated computational approach-combining network toxicology, machine learning (LASSO and XGBoost), bioinformatics, molecular docking, and molecular dynamics simulations-to systematically investigate SDA-induced coagulation dysfunction. We identified 191 potential targets, with significant enrichment in cancer-related pathways, atherosclerosis, and proteoglycans in cancer. Met proto-oncogene (Met) emerged as a core target through machine learning. Analysis of a colorectal cancer dataset (GSE52060) revealed elevated Met expression in patients with coagulation dysfunction, and receiver operating characteristic analysis indicated its strong diagnostic value (area under the curve = 0.856). Molecular docking showed stable binding between SDA and Met (-5.5 kcal/mol), further supported by molecular dynamics simulations demonstrating favorable hydrogen bonding and complex stability. This study provides a theoretical foundation for understanding SDA's role in coagulation dysfunction and supports future preventive and therapeutic strategy development.
Yang et al. (Thu,) studied this question.