Abstract The decline in testicular function with age has raised significant concerns. Long non‐coding RNA (lncRNA) influences a wide array of physiological processes, including spermatogenesis. Nevertheless, the precise roles and regulatory mechanisms of lncRNA in testicular aging remain elusive. This investigation delves into the function of lncRNA:CR43306 in governing spermatogenesis during testicular aging. Depletion of lncRNA:CR43306 in 40‐day‐old Drosophila testes resulted in the hindrance of spermatogenesis, particularly affecting elongated spermatids, leading to functional senescence. Additionally, differentially expressed gene (DEG) expression analysis through bulk RNA‐seq unveiled genes linked to elongated spermatids and cell adhesion. Our findings underscore the pivotal role of lncRNA:CR43306 in testicular aging by influencing cell adhesion. These discoveries illuminate the regulatory pathways of lncRNA in testicular aging and offer valuable insights for potential studies and therapeutic interventions.
Wang et al. (Thu,) studied this question.