Targeted screening of natural thrombin inhibitors from herbal extracts using an enzyme-immobilized microfluidic reactor

Key Points

• The central aim is to evaluate the effectiveness of a microfluidic reactor for screening thrombin inhibitors derived from herbal extracts.
• Developed an enzyme-immobilized microfluidic reactor integrated into a glass-PDMS hybrid chip.
• Conducted in vitro screening of natural compounds for thrombin inhibition.
• Utilized enzyme-catalyzed reactions to assess inhibitory activity.
• Identified several herbal extracts with significant thrombin inhibitory activity.
• Demonstrated the reactor's efficiency in screening potential natural inhibitors.
• Highlighted the potential of herbal extracts in developing new anticoagulants.