Synthesis of Immunotoxin of Anti-CD3 Monoclonal Antibody-Bongkrekic Acid and Cytotoxic Effect Against CD3+ T Cells in Peripheral Blood Mononuclear Cells

Key Points

• The research aims to explore the synthesis of an immunotoxin and its effects on CD3+ T cells.
• Conducted in silico assays to assess interactions between bongkrekic acid and anti-CD3 antibodies.
• Performed in vitro assays to measure cytotoxic effects in peripheral blood mononuclear cells (PBMCs).
• Evaluated the specificity of the conjugate against T cells comparing it to the individual components.
• The conjugates showed lower cytotoxicity compared to bongkrekic acid and anti-CD3 antibody individually.
• In vitro assays indicated that the anti-CD3 MAb exhibited inherent cytotoxic properties in PBMCs.