Investigation of nanoscale topography and biomechanical tuning of PDMS substrates to enhance cardiomyocyte differentiation from human induced pluripotent stem cells

Key Points

• The research aims to investigate how nanoscale topography and biomechanical properties of PDMS substrates affect cardiomyocyte differentiation from human induced pluripotent stem cells (hiPSCs).
• Utilized PDMS substrates with varying micro/nanostructures.
• Assessed cardiomyocyte differentiation from hiPSCs on these substrates.
• Examined the influence of substrate stiffness on cell behavior.
• Improved cardiomyocyte differentiation observed on optimized PDMS substrates.
• Substrate stiffness and nanoscale features significantly influenced iPSC behavior.