Protein migration via dissolution of ATP-stabilized condensate in a gradient of AMP

Key Points

• The aim is to explore how ATP influences protein condensation and migration in crowded environments.
• Utilized bovine serum albumin (BSA) as a model protein.
• Investigated the effects of ATP and AMP on protein behavior.
• Conducted experiments in macromolecularly crowded environments.
• BSA demonstrated an increased tendency to form condensates in ATP's presence.
• AMP gradients promoted the migration of ATP-stabilized condensates.
• Findings suggest a dynamic interaction between ATP and AMP affecting protein behavior.