Stress Inhibits Myogenic Differentiation via Transcriptome Remodeling and GR-Mediated Suppression of the ITGA11-FAK/Paxillin-Akt-GSK3β Cascade

The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.jafc.5c05284 . Primer sequence information (Table S1); details of antibodies (Table S2); siRNA sequence of ITGA11 (Table S3); statistical analysis of transcriptome sequencing sample data (Table S4); number of DEGs in each comparison between groups (Table S5); significantly enriched signaling pathways of 153 key common DEGs (Table S6); cytopathic ratio in SFMs treated with different concentrations of Dex at various differentiation stages (Figure S1); biological process enrichment analysis of DEGs under normal condition (Figure S2); KEGG pathway enrichment analysis of DEGs under normal condition (Figure S3); biological process enrichment analysis of DEGs under stress condition (Figure S4); KEGG pathway enrichment analysis of DEGs under stress condition (Figure S5); comparison between mRNA expression levels (qRT-PCR) and FPKM values (RNA-seq) of randomly selected genes (Figure S6); biological process and KEGG pathway enrichment results of common DEGs under normal differentiation condition (Figure S7); biological process and KEGG pathway enrichment results of common DEGs under stress condition (Figure S8); predicted mechanism diagram of SFM differentiation under normal and stress conditions (Figure S9); cytopathic ratio of SFMs under different concentrations of RU486 (Figure S10); effects of different concentrations of RU486 on Dex-induced inhibition of SFM differentiation (Figure S11); bioinformatics analysis of the sheep ITGA11 protein (Figure S12); amplification and cloning of the CDS region of ITGA11 (Figure S13); detection of ITGA11 overexpression in undifferentiated SFMs under different transfection conditions (Figure S14); cell adhesion ratio of SFMs at different collagen concentrations (Figure S15); detection of ITGA11 overexpression in SFMs at various differentiation stages (Figure S16); siRNA transfection efficiency in SFMs (Figure S17); screening of ITGA11 silencing sites in SFMs (Figure S18); detection of ITGA11 interference effects in undifferentiated SFMs (Figure S19); phenotypic changes in undifferentiated SFMs after ITGA11 interference (Figure S20); detection of ITGA11 interference effects in SFMs at various differentiation stages (Figure S21) ( PDF )