Global diversity analysis of plant-associated Pseudopithomyces fungi reveals a new species producing the toxin associated with facial eczema in livestock: Pseudopithomyces toxicarius sp. nov.

Fig. S1. Phylogenetic tree of 758 genes derived from BUSCO analysis of Illumina whole genome sequences from 146 Pseudopithomyces sequenced isolates in this study. Fig. S2. Bayesian phylogenetic tree of TEF1 sequences showing the relationship of Pse. mori and Pse. entadae to other isolates. Fig. S3. SNP phylogenetic trees and distribution of sporidesmin synthesis in Pse. chartarum isolate and type strains. Fig. S4. SNP phylogenetic trees and distribution of sporidesmin synthesis in Pse. palmicola isolate and type strains. Fig. S5. SNP phylogenetic trees and distribution of sporidesmin synthesis in Pse. toxicarius isolate and type strains. Fig. S6. Comparison of conidial length and width in selected Pseudopithomyces species. Fig. S7. Cardinal temperature growth rates of selected Pseudopithomyces species. Fig. S8. Pseudopithomyces diversisporus alignments of ITS, GAPDH and RPB2 genes. Table S1. Metadata of type species and other isolates, including whole genome sequence accession numbers and sporidesmin synthesis overview. Table S2. HPLC analysis of sporidesmin synthesis. Table S3. LC-MS analysis of sporidesmin synthesis. Table S4. Whole genome sequencing, assembly and BUSCO outputs. Table S5. Amino acid identity of Spd genes in Pse. toxicarius and Pse. chartarum.