To investigate the protective effects of vitamin C (VitC) against oxygen–glucose deprivation/reoxygenation (OGD/R) injury and its underlying mechanisms involving oxidative stress and inflammation in oligodendrocyte lineage cells and microglia. Primary rat oligodendrocyte precursor cells (OPCs), mature oligodendrocytes (OLs), and BV2 microglia were subjected to OGD/R with or without VitC. Apoptosis (TUNEL), differentiation (MBP⁺/PDGFRα⁺), oxidative stress (ROS, MDA), and microglial cytokine profiles (qRT-PCR/ELISA) were assessed. Co-culture experiments were conducted in transwell systems using primary rat OPCs or mature OLs seeded in the upper inserts and BV2 microglia seeded in the lower chambers, allowing paracrine communication without direct cell-cell contact. Biological replicates: n = 3–5 litters for primary cells; ≥3 passages for BV2. OGD/R increased apoptosis in OPCs(P 0.05). VitC (5 µg/mL) protects oligodendrocyte lineage cells via direct antioxidant effects and bidirectional modulation of microglial inflammation, supporting its potential as a multi‑target agent for white‑matter disorders.
Guo et al. (Wed,) studied this question.