A novel loop-mediated isothermal amplification assay coupled with lateral flow assay for differential detection of Brucella spp from Brucella abortus S19 vaccine strain

Key Points

• Novel loop-mediated isothermal amplification assay successfully detects Brucella spp with high specificity.
• The method provides differential detection of the Brucella abortus S19 vaccine strain and wild-type strains.
• Assessment using a lateral flow assay enhances the ease of interpretation and rapid results for detection.
• The findings highlight potential clinical applications in diagnosing Brucella infections effectively.