Nanopore sequencing of the Tunisian gut microbiome: effect of the DNA extraction methods

Key Points

• Nanopore sequencing revealed significant differences in microbial diversity depending on the DNA extraction method used.
• The highest diversity was observed with a specific extraction technique, showing a notable increase in certain microbial populations.
• Analysis of gut microbiome was conducted through metagenomics with samples collected across various regions of Tunisia.
• Findings highlight the crucial role of DNA extraction in accurately profiling gut microbiome features.