March 3, 2026Open Access

High‐throughput single‐cell DNA methylation and chromatin accessibility co‐profiling with SpliCOOL‐seq

Key Points

SpliCOOL-seq uncovers genes tied to tumorigenesis, revealing new insights into LUAD mechanisms.
DNMT inhibitors triggered demethylation in cancer cells, leading to diverse epigenetic responses.
Analysis of single-cell profiles provides comprehensive data on DNA methylation and chromatin accessibility.
Potential implications for understanding age-related and cancer-related epigenetic changes are significant.

Abstract

SpliCOOL-seq achieves high-throughput single-cell co-profiling of DNA methylation and chromatin accessibility. DNMT inhibitors caused cancer cell demethylation with divergent patterns. SpliCOOL-seq enables the discovery of genes related to LUAD tumorigenesis. Ageing and LUAD tumorigenesis may share similar epigenetic alterations.

High‐throughput single‐cell DNA methylation and chromatin accessibility co‐profiling with SpliCOOL‐seq

Key Points

Abstract

Cite This Study