Genetic Transformation in Brassica juncea L. using Wild Types and Shooter Mutants of Agrobacterium tumefaciens and the Regeneration Response

Key Points

• Genetic transformation of Brassica juncea was successfully achieved with a focus on regeneration response.
• The use of wild types and shooter mutants of Agrobacterium tumefaciens showed significant effects in transformation rates.
• Assessment used various experimental setups to evaluate regeneration response under different conditions.
• This study highlights the potential for improving Brassica juncea through tailored agrobacterium mutations.