• First LC-MS/MS characterization of sulfated metabolites in C. lancifolius leaves. • Ten sulfated compounds identified, including flavonoids, phenolic acids, fatty alcohols, and a jasmonate derivative. • Neutral loss of SO 3 (80 Da) was a key diagnostic feature for structural interpretation. • Sulfated flavonoids dominated the metabolic profile, indicating active sulfur-conjugation pathways. • Presence of sulfated metabolites in C. lancifolius grown in Saudi Arabia suggest roles in environmental stress adaptation. Sulfated metabolites, particularly sulfated flavonoids, represent a unique subclass of plant secondary metabolites that have gained increasing attention due to their diverse biological activities and ecological roles. Little is known about the existence and characteristics of sulfated flavonoids in Conocarpus species, particularly those cultivated in Saudi Arabia under harsh environmental stress conditions. In this study, the sulfated metabolome of C. lancifolius leaves was investigated for the first time using LC-MS/MS in the negative ion mode, enabling the tentative identification of ten sulfated metabolites, including five flavonoid sulfates, two sulfated fatty alcohols, two phenolic acid sulfates, and the phytohormone derivative 12-sulfoxyjasmonic acid. The mass fragmentation profiles of syringetin-3-sulfate and 1,2-tetradecanediol 1-(hydrogen sulfate), two tentatively identified metabolites, were presented here for the first time. The MS/MS fragmentation patterns were dominated by the neutral loss of SO 3 , producing characteristic aglycone or backbone-related ions that supported structural assignments. The diversity of sulfated metabolites detected in C. lancifolius indicated that sulfate conjugation plays a substantial role in the secondary metabolism of the plant, potentially contributing to adaptive responses to environmental stress. These findings provided a groundwork for understanding the sulfur-associated metabolome of this species and underscore the need for further phytochemical, physiological, and pharmacological investigations.
Bar et al. (Tue,) studied this question.