Development and evaluation of RT-qPCR assays for detection of Alagoas vesiculovirus (VSAV) considering the genetic diversity of Brazilian isolates

Key Points

• Detection of Alagoas vesiculovirus improves with newly developed RT-qPCR assays across various genetic isolates.
• The assays demonstrated high sensitivity and specificity to different Brazilian isolates of vesiculovirus.
• Assessment involved evaluating genetic diversity through comprehensive analysis of unique Brazilian strains.
• Findings support enhanced viral detection, with broader implications for virology and public health in Brazil.