To address the stability and cost limitations of enzymatic glucose sensors, this work details the use of nanoporous copper (NPC) modified microdisc array (MDA) sensors for non-enzymatic glucose detection, with a focus on bovine health monitoring. Electrochemical analysis in 0.1 M NaOH, using cyclic voltammetry (CV) and chronoamperometry (CA), demonstrated excellent sensor performance. The device demonstrated a wide linear detection range (0.01 – 4.0 mM, R 2 >0.99) and a 4 µM limit of detection (LOD). It achieved high sensitivities of 14.87 and 9.87 µA µM -1 cm -2 across its low-concentration and full linear detection ranges, respectively. The sensor showed high selectivity, with minimal interference from common analytes in saliva and blood, and ageing studies revealed stable performance for up to 56 days when stored in acetone. Successful validation in artificial saliva confirmed the sensor's applicability for direct biofluid analysis. These findings establish NPC-modified MDAs as a robust, cost-effective, and promising platform for glucose monitoring in complex biofluids, particularly for veterinary diagnostics and agri-tech applications. • Nanoporous copper microdisc arrays (NPC MDAs) were developed for non-enzymatic glucose sensing for bovine health monitoring. • The sensor has a wide linear detection range of 0.01 to 4 mM and a low limit of detection of 4 µM. • It achieved a high sensitivity of 14.87 and 9.87 µA µM -1 cm -2 across its low-concentration (0-0.1 mM) and full linear detection ranges, respectively. • The sensor demonstrated high selectivity against common saliva - and blood-based analytes and was resilient to chloride poisoning. • A stable operational lifespan of 28 days in air and 56 days in acetone was established. • Its effectiveness was successfully validated for glucose detection in artificial saliva.
Dixon et al. (Fri,) studied this question.