Single-Cell Analysis A single cell and barcoded beads are entrapped within a permeable hydrogel. The barcoded beads capture proteins secreted by the cell using antibodies, while the hydrogel allows seamless diffusion of labeled antibodies. Jointly, they empower a multiplex sandwich immunoassay at the single cell level that is quantified by microscopy. More details can be found in the Research Article by Félix Lussier, David Juncker, and co-workers (DOI: 10.1002/adma.202506398).
Lussier et al. (Sun,) studied this question.