Aims: Medicinal plants are renowned for their pharmacological attributes in combating diverse pathogens.While these plants have historically been employed to treat diseases and inflammations, the pathogenic microorganisms, such as Escherichia coli, Salmonella typhi, Bacillus anthracis inherent in them pose potential health risks.This study evaluates the effectiveness of cobalt-60 gamma irradiation in reducing microbial contaminants in Labisia pumila (Kacip Fatimah).Methodology and results: Different radiation doses (3, 6, 9, and 12 kGy) were administered, and microbial contents was analyzed using microbiological analyses, Total Aerobic Microbial Counts (TAMC) and Total Yeast Mold Counts (TYMC).The pathogenic microorganisms were first phenotypically identified using selective media and further confirmed through PCR amplification.Besides, qualitative screening using chemical tests was performed to identify the phytochemical contents.The findings demonstrated a significant reduction in total microbial load after a 3 kGy dose (P < 0.05, MANOVA), whereas higher doses did not lead to further substantial reductions.Importantly, no pathogenic bacteria were detected in the plant after a 9 kGy dose, and the constituents were maintained. Conclusion, significance and impact of study:This study confirms that cobalt-60 gamma irradiation effectively reduces microbial contamination in L. pumila while preserving its phytochemicals.The optimal 9 kGy dose enhances herbal safety and supports its use in quality control and regulation.These findings reinforce irradiation as a reliable decontamination method for medicinal plants.
Jamal et al. (Sun,) studied this question.