Screening of Y‐chromosomal STR loci and development of a multiplex PCR system for paternal lineage identification in horses

Key Points

• The aim is to identify new Y-STR loci for effective paternal lineage identification in horses.
• Identified fifteen new Y-STR loci in horses.
• Assessed polymorphism in three Y-STR loci.
• Developed a multiplex PCR system to test these loci.
• Fifteen new Y-STR loci were successfully identified.
• Three of these loci showed polymorphism, enhancing genetic diversity assessment.
• The multiplex PCR system allows reliable identification of horse paternal lines.