This study evaluated the effects of Ginkgo biloba leaf extract (GBE) on Exopalaemon carinicauda reared under low salinity. Shrimps were fed diets supplemented with 0.0 (control), 1.0, 2.0, or 4.0 g/kg GBE for 30 days. The results indicated that, while GBE did not significantly enhance growth performance, it markedly improved several physiological parameters. Supplementation at 1.0–2.0 g/kg GBE did not affect hemolymph ALT or AST activities ( P > 0.05), 4.0 g/kg dose resulted in a significant increase ( P 0.05). Notably, the expression of fas exhibited a biphasic response, with initial up-regulation at doses of 1.0 and 2.0 g/kg followed by a significant decline at the 4.0 g/kg dose ( P < 0.05). Concurrently, GBE markedly upregulated the expression of the lipolytic genes lps and hsl ( P < 0.05), consistent with the decrease of hemolymph lipid. GBE downregulated the expression of cyp2l1 , cyp2u1 , cyp3a25 , and cyp4c ( P < 0.05). Conversely, it markedly upregulated the expression of cyp6a8 , cyp9e2 , and cyp12a2 ( P < 0.05). In summary, despite not improving growth performance of E. carinicauda , 1.0–2.0 g/kg GBE exerted beneficial effects on antioxidant status, immune function, and lipid metabolism. The optimal recommended level of GBE supplementation is 2.0 g/kg in the diet of E. carinicauda . • Dietary supplementation with GBE significantly increased the population of blister-like cells. • 1.0–2.0 g/kg GBE exerted beneficial effects on antioxidant status, immune function, and lipid metabolism. • The optimal recommended inclusion level of GBE supplementation is 2.0 g/kg.
Liang et al. (Sat,) studied this question.