An Orthogonal Extraction Workflow for High-Depth Plasma Proteomics with Metabolomic Compatibility from Low Microliter-Scale Samples

Key Points

• To develop a workflow that enhances detection of low-abundance proteins in plasma for biomarker discovery and clinical application.
• Developed an orthogonal extraction workflow for plasma samples.
• Tested the method on low microliter-scale plasma samples.
• Integrated metabolomic compatibility to streamline analysis.
• Improved detection rates of low-abundance proteins by the new workflow.
• Enhanced signal clarity, reducing overlaps in similar disease profiles.
• Facilitated better identification of potential clinical biomarkers.