A general chemistry laboratory experiment that can be completed in a single laboratory period is described that familiarizes students with the acid–base chemistry of a diprotic acid and with the use of visible spectroscopy to determine species concentrations. This experiment is a modified version of a previously described laboratory exercise developed for an upper-division quantitative analysis course. Students work in teams and as a class to generate different ionization states of various highly absorbing dyes. Both spectroscopic and potentiometric (pH) data is collected using LabWorks II stations, but other inexpensive pH meters and visible spectrometers (e.g., Spec 20s) are suitable. A spreadsheet template is used to determine the percent composition of various ionization states of a diprotic acid and to determine the pKa values. Besides introducing students to fundamental tools and key chemical concepts, this laboratory is also inexpensive to operate and utilizes nontoxic, colorful solutions.
Smith et al. (Fri,) studied this question.
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