The relevance of the study is due to the high frequency of drug-induced liver injury in mammals and insufficient study of the issue of amino acid metabolism disorders in acute forms of hepatopathy of the corresponding genesis. In this regard, the study was aimed at identifying specific changes in the profile of free amino acids in the bile and liver of rats with tetracycline-induced fatty hepatosis, as well as determining the corrective effect of the phospholipid fraction of milk. The leading approach in studying this problem was an experiment on laboratory animals with modelling of fatty hepatosis due to the cytotoxic effect of tetracycline, followed by the selection of bile and liver samples, followed by studying the spectrum of free amino acids using thin-layer chromatography with ninhydrin staining. It was found that the general pattern of changes in the hepatobiliary system of diseased rats indicated a violation of bile acid conjugation processes and mitochondrial dysfunction, as well as a pronounced blockage of the use of free amino acids in metabolic processes. At the same time, the use of phospholipid-containing therapy in rats with tetracycline-induced fatty hepatosis activated the use of the intrahepatic reserve of amino acids involved in bile acid conjugation and antioxidant protection, in particular sulphur-containing representatives and the total fraction of glycine/serine/glutamine. It was determined that the bile of these rats also had a reduced content of glycine and taurine-containing amino acid fractions in the bile of these rats, with a simultaneous increase in the level of the total alanine/tyrosine/threonine and arginine/ornithine/lysine fractions, confirming the activation of detoxification processes in the liver involving free amino acids. It was found that the use of the phospholipid fraction of milk reduced the manifestations of disturbances in the intermediate metabolism of amino acids and contributed to the restoration of the content of leucine- and valine-containing fractions in the bile and liver of sick rats. The established patterns are of practical value for laboratory diagnosis and preclinical evaluation of hepatoprotective agents, since the amino acid profile of the hepatobiliary system can be used as a sensitive indicator of the severity of liver injury and as a criterion for determining the effectiveness of therapy
Bryzhenko et al. (Mon,) studied this question.