An efficient strategy was developed for the preparation of chemically modified carbon paste electrodes (CPE) using graphene oxide (GO) to promote the desired interaction of the antimicrobial Peptide Tritrpticin (TRP3) with them. This interaction was chosen considering the important study on the biological antimicrobial activity and the remarkable sensory capacity of GO and TRP3. The interaction of the chemically modified electrode, graphene oxide associated with tripticin (GOTRP3), was characterized by FTIR and electrochemical techniques, including cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). FTIR analysis revealed changes in the GO and GOTRP3 samples and exhibited bands corresponding to functional polar groups and C-N stretching mode. The CV results confirmed that the GO samples facilitated the interaction of TRP3 with the CPE by promoting the adsorption of TRP3 at its oxidized defect sites. In the presence of potassium ferricyanide, the oxidation peak potential showed a negative shift of 20mV, indicating facilitated electron transfer between GO and TRP3. EIS measurements evidenced the interaction of TRP3 with GO and showed a lower electron transfer resistance, which was evident in the almost linear part of the Nyquist plot compared to the CPE samples. In addition, UV/VIS spectrophotometry was performed to analyze the antimicrobial activity of TRP3 and TRP3GO in vitro with the interaction of Enterococcus faecalis (ATCC 31299). The method used was agar Mueller-Hinton. The results showed that GO TRP3 inhibited the antimicrobial activity of ATCC 31299 in the center of the gentamicin probe by 16.96% more than TRP3 after 24 hours of incubation. The potential of GOTRP3 to inhibit antimicrobial activity increased to 27.4% after 48 hours. The biocompatibility and antimicrobial properties of graphene oxide and tritrpticin were studied. GO and TRP3 were found to have low toxicity. GOTRP3 showed cell viability above 80% at all concentrations, suggesting that GO acts as a controlled release system for TRP3. Statistical analysis confirmed significant differences between concentrations for cell viability, reinforcing a dose-dependent profile for TRP3 and GO separately, but not for GOTRP3. This suggests that GOTRP3 is biocompatible and has potential for biomedical applications, exhibiting enhanced antimicrobial effects while maintaining cellular safety.
Jesus et al. (Wed,) studied this question.