Repeated administration of diazepam at 5 mg/kg for 5 days significantly reduced cardiac functional recovery during reperfusion and exacerbated ischemia-reperfusion injury in isolated rat hearts.
Does repeated diazepam administration affect cardiac performance and injury in ischemia-reperfused isolated rat hearts?
Repeated administration of diazepam exacerbates ischemia-reperfusion injury and reduces cardiac performance in an isolated rat heart model.
Absolute Event Rate: 29.98% vs 53.88%
p-value: p=0.004
OBJECTIVE: To evaluate whether repeated diazepam administration affects the heart in ischemia- reperfusion. METHODS: This study was performed at the Medical Biology Research Center, Kermanshah, Iran, from March to September 2008. Four groups of rats were subjected to a daily injection of diazepam (group 1 0.5 mg/kg for 21 days, group II 2.5 mg/kg for 5 days, and group III 5 mg/kg for 5 days intraperitoneally), and saline solution (21 days) in the control groups. Isolated, perfused hearts were subjected to 40 minutes global ischemia, and 45 minutes reperfusion. The left ventricular developed pressure (LVDP), heart rate, and coronary flow were measured. Rate pressure product (RPP) was calculated. In reperfusion, released lactate dehydrogenase (LDH) enzyme in effluent was measured. RESULTS: It was observed that the recovery of the RPP and LVDP in reperfusion significantly decreased in the test group III (n=9) in comparison to the control (n=8). During the reperfusion period, the released LDH significantly increased in test group II (n=8) and group III in comparison with the control. CONCLUSION: The results show that repeated administration of diazepam (5 mg/kg for 5 days) reduced the cardiac performance in reperfusion, and significantly exacerbated the ischemia-reperfusion injury. It is probably mediated by the changing of cardiac susceptibility in ischemia due to repeated administration of diazepam. The results show that repeated administration of diazepam (5 mg/kg for 5 days) reduced the cardiac performance in reperfusion, and significantly exacerbated the ischemia-reperfusion injury. It is probably mediated by the changing of cardiac susceptibility in ischemia due to repeated administration of diazepam.
Shackebaeit et al. (Mon,) conducted a other in Ischemia-reperfusion injury (n=32). Diazepam vs. Saline solution was evaluated on Recovery percentage of rate pressure product (RPP) at 45th minute of reperfusion (p=0.004). Repeated administration of diazepam at 5 mg/kg for 5 days significantly reduced cardiac functional recovery during reperfusion and exacerbated ischemia-reperfusion injury in isolated rat hearts.