Extended Characterisation of the Coding Sequence of the HLA‐A*24:167 Allele by Next‐ and Third‐Generation Sequencing

Key Points

• To characterize the coding sequence of the HLA-A*24:167 allele and compare it with HLA-A*24:22.
• Utilized next-generation sequencing
• Employed third-generation sequencing techniques
• Focused on the detection of nucleotide substitutions
• Identified a single nucleotide substitution in codon 158
• Compared the sequence variation between HLA-A*24:167 and HLA-A*24:22
• Confirmed the presence of this variation within exon 3