Uncovering the copper(I) binding abilities of a unique fungal metallothionein: Characterization of Yarrowia lipolytica MT and its Y54C mutant

Key Points

• To investigate the copper(I) binding abilities of the fungal metallothionein from Yarrowia lipolytica and its Y54C mutant.
• Characterization of metallothioneins using biochemical assays
• Comparison of copper binding between wild-type and Y54C mutant
• Analysis of metal detoxification mechanisms
• Fungal metallothionein demonstrated significant copper(I) binding
• Y54C mutant showed altered binding ability compared to the wild type
• Findings suggest potential applications in bioremediation.