RNA-binding Landscape of Amiloride: Large-scale Profiling and Structural Basis of U–U Mismatch Recognition

Key Points

• Investigate how amiloride interacts with RNA, focusing on its ability to recognize uracil and U–U mismatches.
• Conducted large-scale profiling of amiloride's binding properties
• Analyzed structural interactions using advanced techniques
• Evaluated selectivity towards various RNA motifs
• Identified specific binding sites on RNA for amiloride
• Confirmed that amiloride can recognize U–U mismatches effectively
• Revealed the importance of hydrogen bonding in binding selectivity