Backbone N-Heteroatom Substitution as a Strategy to Enhance Peptide Proteolytic Stability

Key Points

• This research aims to explore how modifying the backbone of peptides can enhance their stability against proteolytic degradation.
• Employing backbone N-heteroatom substitutions in peptide design.
• Assessing stability through proteolytic degradation assays in biological environments.
• Comparative analysis of modified and unmodified peptides.
• Significant increase in proteolytic stability for peptides with N-heteroatom substitutions compared to controls, p<0.01.
• Enhanced binding affinity observed for modified peptides on biomolecular surfaces.
• Reduction in protease recognition leading to sustained activity in biological trials.