Bioenzyme-free dual-mode sensing for glyphosate based on fluorescence Fe-MOF nanozyme

Key Points

• This study aims to develop a sensitive, dual-mode detection method for glyphosate using a fluorescent nanozyme.
• Constructed a dual-mode sensing strategy based on MIL-101-NH2(Fe) for glyphosate detection.
• Utilized peroxidase-like activity of the nanozyme to catalyze OPD oxidation.
• Developed a portable detection swab combined with a smartphone for practical applications.
• Achieved linear ranges of 100–1500 ng/mL and 25–3000 ng/mL for colorimetric and ratio fluorescence methods, respectively.
• Detection limits were established at 75 ng/mL and 20 ng/mL for colorimetric and fluorescence assays, respectively.
• Recovery rates for actual samples were between 92.0% and 102.0%.