Click Reaction-Mediated Ligation of Split Guide DNA to Activate Clostridium butyricum Argonaute for Amplification-Free Detection of Escherichia coli O157:H7.

Key Points

• The aim is to develop a fast and efficient method for detecting Escherichia coli O157:H7 using DNA ligation techniques.
• Utilized click reaction for ligation of split guide DNA.
• Activated Clostridium butyricum Argonaute for detection purposes.
• Evaluated the method using real samples.
• Achieved successful detection of Escherichia coli O157:H7 in real samples.
• Demonstrated quick and effective results without amplification steps.