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BACKGROUND: Sugar phosphates are important intermediates of central carbon metabolism in biological systems, with roles in glycolysis, the pentose-phosphate pathway, tricarboxylic acid (TCA) cycle, and many other biosynthesis pathways. Understanding central carbon metabolism requires a simple, robust and comprehensive analytical method. However, sugar phosphates are notoriously difficult to analyze by traditional reversed phase liquid chromatography. RESULTS: leaf extracts, determined by a two-level spiking approach of selected metabolites, was 79-107%. CONCLUSION: extracts.
Rende et al. (Thu,) studied this question.
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