Background: Natural bioactive compounds offer therapeutic and cosmetic benefits but are often limited by low stability and poor skin penetration. Liposomal nanocarriers represent a promising strategy to overcome these barriers 1. Objective: Optimize liposomes encapsulating an extract from Actinidia arguta fruit for topical use, with potential relevance in antitumor skin therapies. Methods: The extract was obtained by ultrasound-assisted extraction 2 and incorporated into phospholipid liposomes via probe sonication 3. A central composite design was applied to optimize lecithin, extract concentration, and sonication amplitude, targeting minimal vesicle size (VS) and polydispersity index (PDI), and maximal encapsulation efficiency (EE). The optimized liposomes were characterized regarding phytochemical composition (LC-DAD-MS), structural integrity (TEM, FTIR, DSC) and stability. Biocompatibility was investigated by measuring cell viability of HDFa, HaCaT, and A375 cells after 24 h of exposure using the MTT assay. Results: The optimized liposomes consisted of 167.9 mg/mL lecithin, 42.5 mg/mL extract, and 28% amplitude (R² = 0.995), yielding nanosized vesicles (107.2±2.2 nm) with uniform distribution (PDI of 0.173±0.011), a strongly negative zeta potential (ZP; −47.8±1.6 mV), and an EE of 50.7±2.5%. LC-DAD-MS confirmed the presence of chlorogenic and neochlorogenic acids, catechin, kaempferol, quercetin, and sugar derivatives. Over 90 days, liposomes remained stable (VS70% viability in keratinocytes and fibroblasts, with mild stimulation at low doses. A375 melanoma cells exhibited slight, dose-dependent reductions in viability only at high concentrations, suggesting nonspecific metabolic effects related to the lipid vesicles or the sugars present on the extract. Conclusions: The optimized liposomal system efficiently encapsulates A. arguta fruit antioxidants, while maintaining stability and safety, supporting its suitability for topical delivery and further evaluation in melanoma models. Figure 1. Characterization of optimized liposomes: (A) TEM images of loaded (left) and unloaded liposomes (right), (B) DSC thermograms, (C) FTIR spectra and (D) HaCaT, HDFa and A375 cell viability after 24h of exposure (n = 3); *p < 0.05.
Teixeira et al. (Sat,) studied this question.