The protein kinase FaSnRK1α regulates sucrose accumulation in strawberry fruits

In strawberry, sucrose is the major form of carbohydrate translocated from the leaves to the fruits and plays an important role in fruit ripening. As a conserved energy sensor, sucrose nonfermenting-1 (SNF1)-related kinase 1 (SnRK1) plays an important role in plant carbon metabolism. However, evidence that SnRK1 regulates sucrose accumulation in fruits is lacking. In this study, we transiently expressed FaSnRK1α in strawberry fruits and found that overexpression (OE) of the FaSnRK1α gene significantly increased the sucrose content, whereas repression of FaSnRK1α by RNA interference (RNAi) decreased the sucrose content. Further analysis revealed that FaSnRK1α increased the expression of FaSUS1 and FaSUS3 as well as the activity of sucrose synthase (SUS; EC 2.4.1.13) and that FaSPS1 expression and sucrose phosphate synthase (SPS; EC 2.4.1.14) activity were strongly downregulated, which decreased the accumulation of sucrose. However, the expression of FaSPS3, which is reported to contribute to sucrose accumulation, was induced by FaSnRK1α, and FaNI expression and invertase (INV; EC 3.2.1.26) activity were upregulated by FaSnRK1α. In addition, FaSnRK1α positively upregulated the expression of the sucrose transporter (SUT) genes FaSUT1 and FaSUT5 and interacted with FaSUS1, FaSPS1 and FaSPS3 proteins but not with FaSUS3, FaNI, FaSUT1 or FaSUT5 proteins. Overall, FaSnRK1α systematically regulates the expression of the genes and activities of key enzymes involved in the sucrose metabolic pathway and promotes the long-distance transport of sucrose, thereby increasing sucrose accumulation and ultimately promoting fruit ripening. However, the mechanisms by which sucrose transport and degradation are regulated by SnRK1 warrant additional research.