The mRNA architecture of the translation termination site primes programmed stop codon readthrough events in Drosophila

Key Points

• This research aims to explore how the architecture of mRNA at translation termination sites affects stop codon readthrough events in Drosophila.
• Utilized computational models to predict mRNA stem-loop structures associated with SCR.
• Refined prediction models to assess the effectiveness of stem-loops in promoting SCR of heterologous mRNAs.
• Identified specific mRNA structures that significantly enhance SCR events.
• Demonstrated that the architecture of the termination site plays a critical role in modulating readthrough efficiency.