Abstract Background and aims Arm weakness is common after stroke. Movement kinematics could identify intervention targets but are currently unavailable to clinicians. We evaluated accessible video-based markerless motion capture (MMC) accuracy for upper-limb kinematics in hemiplegic stroke survivors. Methods Thirteen stroke survivors (male/female: 7/5, age: 62.3 (6.2) years, recruitment ongoing) performed a drinking task. Eleven were included in the affected arm analysis and were grouped by Fugl–Meyer Upper Limb score: mild (51–60, n=2), moderate (26–50, n=5), and severe (0–25, n=4). Motion data was recorded concurrently using Vicon (120 Hz) and MMC, using two RealSense RGB-depth cameras (30 Hz) and RTMPose. Shoulder, elbow, and wrist angles and normalised wrist tangential velocity (TVW) were computed for affected and unaffected arms. Agreement was assessed using RMSE, Concordance Correlation Coefficient (CCC), and Pearson’s r. Results Fig 1 presents group-based results. Across groups, shoulder and elbow flexion showed strong similarity (r=0.90–0.99) with moderate RMSE (shoulder 6.4–12.5°, elbow 11.7–13.4°) and variable CCC (shoulder 0.52–0.94; elbow 0.08–0.96). TVW showed the best agreement (RMSE 0.1–0.3; CCC 0.89–0.97; r 0.87–0.96). Wrist flexion showed the poorest agreement, especially in moderate/severe stroke (RMSE 11.6–20.1°; CCC 0.14–0.63; r 0.24–0.82). Shoulder abduction varied in relation to severity (RMSE 2.8–6.5°; CCC 0.47–0.90; r 0.72–0.95). Conclusions Accessible MMC techniques show promise for quantifying post-stroke upper-limb movement, but agreement varies between joints and declines with impairment. Performance could be improved through alternative camera setups and optimised MMC approaches. Conflict of interest Rylea Hart: Funding provided by Private Physiotherapy Educational Fund (PPEF) Scheme C Award 2024 and the Physiotherapy Research Fund Award PRF /24A/POD01. No further disclosures. Sera Boston: Funding provided by Private Physiotherapy Educational Fund (PPEF) Scheme C Award 2024. No further disclosures. Letizia Gionfrida: Nothing to disclose. Irene Di Giulio: Nothing to disclose. Ulrike Hammerbeck: Funding provided by Private Physiotherapy Educational Fund (PPEF) Scheme C Award 2024 and the Physiotherapy Research Fund Award PRF /24A/POD01. No further disclosures. Figure 1 - belongs to Results
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