Objective: This study investigated the anti-amnesic effects of Jasminum sambac ethanolic leaf extract in scopolamine-induced amnesic rats, using Donepezil (5 mg/kg) as the reference standard.Material and Method: The ethanolic extract was prepared by maceration and characterized through qualitative and quantitative phytochemical analyses. GC–MS analysis was performed to identify bioactive constituents. Cognitive function was evaluated using the elevated plus maze (EPM), hole board test, Morris water maze (MWM), and actophotometer. Biochemical parameters, including acetylcholinesterase (AChE) activity and antioxidant status, were also assessed.Result and Discussion: Phytochemical screening confirmed the presence of flavonoids, phenols, anthraquinones, saponins, and coumarins, with total phenolic (0.29 mg GAE/g leaf powder) and flavonoid (0.39 mg QE/g leaf powder) contents. The DPPH assay showed 62.55% inhibition at 200 µg/ml (IC₅₀: 109.38 µg/ml). GC–MS identified 29 compounds, including six with antioxidant potential. Behavioral studies demonstrated significant memory enhancement (p 0.05), evidenced by reduced escape latency (MWM), decreased transfer latency (EPM), increased exploratory behavior, and improved locomotor activity. The findings suggest neuroprotective effects mediated via AChE inhibition and attenuation of oxidative stress, supporting further investigation for cognitive impairment and Alzheimer’s disease management.
Mali et al. (Thu,) studied this question.