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Formalin-fixed paraffin-embedded (FFPE) tissue specimens comprise a potentially valuable resource for retrospective biomarker discovery studies, and recent work indicates the feasibility of using shotgun proteomics to characterize FFPE tissue proteins. A critical question in the field is whether proteomes characterized in FFPE specimens are equivalent to proteomes in corresponding fresh or frozen tissue specimens. Here we compared shotgun proteomic analyses of frozen and FFPE specimens prepared from the same colon adenoma tissues. Following deparaffinization, rehydration, and tryptic digestion under mild conditions, FFPE specimens corresponding to 200 µg of protein yielded ∼400 confident protein identifications in a one-dimensional reverse phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The major difference between frozen and FFPE proteomes was a decrease in the proportions of lysine C-terminal to arginine C-terminal peptides observed, but these differences had little effect on the proteins identified. No covalent peptide modifications attributable to formaldehyde chemistry were detected by analyses of the MS/MS datasets, which suggests that undetected, cross-linked peptides comprise the major class of modifications in FFPE tissues. Fixation of tissue for up to 2 days in neutral buffered formalin did not adversely impact protein identifications. Analysis of archival colon adenoma FFPE specimens indicated equivalent numbers of MS/MS spectral counts and protein group identifications from specimens stored for 1, 3, 5, and 10 years. Combination of peptide isoelectric focusing-based separation with reverse phase LC-MS/MS identified 2554 protein groups in 600 ng of protein from frozen tissue and 2302 protein groups from FFPE tissue with at least two distinct peptide identifications per protein. Analysis of the combined frozen and FFPE data showed a 92% overlap in the protein groups identified. Comparison of gene ontology categories of identified proteins revealed no bias in protein identification based on subcellular localization. Although the status of posttranslational modifications was not examined in this study, archival samples displayed a modest increase in methionine oxidation, from ∼17% after one year of storage to ∼25% after 10 years. These data demonstrate the equivalence of proteome inventories obtained from FFPE and frozen tissue specimens and provide support for retrospective proteomic analysis of FFPE tissues for biomarker discovery. Formalin-fixed paraffin-embedded (FFPE) tissue specimens comprise a potentially valuable resource for retrospective biomarker discovery studies, and recent work indicates the feasibility of using shotgun proteomics to characterize FFPE tissue proteins. A critical question in the field is whether proteomes characterized in FFPE specimens are equivalent to proteomes in corresponding fresh or frozen tissue specimens. Here we compared shotgun proteomic analyses of frozen and FFPE specimens prepared from the same colon adenoma tissues. Following deparaffinization, rehydration, and tryptic digestion under mild conditions, FFPE specimens corresponding to 200 µg of protein yielded ∼400 confident protein identifications in a one-dimensional reverse phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The major difference between frozen and FFPE proteomes was a decrease in the proportions of lysine C-terminal to arginine C-terminal peptides observed, but these differences had little effect on the proteins identified. No covalent peptide modifications attributable to formaldehyde chemistry were detected by analyses of the MS/MS datasets, which suggests that undetected, cross-linked peptides comprise the major class of modifications in FFPE tissues. Fixation of tissue for up to 2 days in neutral buffered formalin did not adversely impact protein identifications. Analysis of archival colon adenoma FFPE specimens indicated equivalent numbers of MS/MS spectral counts and protein group identifications from specimens stored for 1, 3, 5, and 10 years. Combination of peptide isoelectric focusing-based separation with reverse phase LC-MS/MS identified 2554 protein groups in 600 ng of protein from frozen tissue and 2302 protein groups from FFPE tissue with at least two distinct peptide identifications per protein. Analysis of the combined frozen and FFPE data showed a 92% overlap in the protein groups identified. Comparison of gene ontology categories of identified proteins revealed no bias in protein identification based on subcellular localization. Although the status of posttranslational modifications was not examined in this study, archival samples displayed a modest increase in methionine oxidation, from ∼17% after one year of storage to ∼25% after 10 years. These data demonstrate the equivalence of proteome inventories obtained from FFPE and frozen tissue specimens and provide support for retrospective proteomic analysis of FFPE tissues for biomarker discovery. Formalin-fixed paraffin-embedded (FFPE) 1The abbreviations used are:FFPEformalin-fixed paraffin-embeddedIHCimmunohistochemistryPMpyridoxamineIDidentificationIEFisoelectric focusingFAformic acidACNacetonitrileFDRfalse discovery ratesANOVAanalysis of variancePTMposttranslational modification. tissue samples are routinely prepared during the pathological characterization of clinical specimens and are abundantly available in pathology archives worldwide. The fixation process yields clinically relevant samples that can be stored at ambient temperature and are suitable for pathological examination by light microscopy even after years in storage. Given the wealth of clinical data associated with specimens collected over a span of decades, such as patient treatment regimens and outcomes, FFPE tissue represents a potentially valuable resource for biomarker discovery through retrospective analysis (1Hood B.L. Conrads T.P. Veenstra T.D. Mass spectrometric analysis of formalin-fixed paraffin-embedded tissue: unlocking the proteome within.Proteomics. 2006; 6: 4106-4114Crossref PubMed Scopus (84) Google Scholar, 2Yamashita S. Heat-induced antigen retrieval: mechanisms and application to histochemistry.Prog. Histochem. Cytochem. 2007; 41: 141-200Crossref PubMed Scopus (125) Google Scholar). formalin-fixed paraffin-embedded immunohistochemistry pyridoxamine identification isoelectric focusing formic acid acetonitrile false discovery rates analysis of variance posttranslational modification. However, fixation of tissue in formalin leads to significant cross-linking among proteins and other biomolecules, rendering the samples incompatible with many biochemical analyses. Immunohistochemical (IHC) analysis of FFPE tissue has been conducted since the 1970s using either proteolysis or protein denaturants to expose antigenic regions of proteins (3Huang S.N. Minassian H. More J.D. Application of immunofluorescent staining on paraffin sections improved by Google Scholar, S. H. S. of in the of and PubMed Scopus Google Scholar). the of in FFPE tissue has been improved through the of antigen in paraffin-embedded for staining based on of tissue Histochem. Cytochem. PubMed Scopus Google Scholar, S. the of formalin fixation with and a antigen Histochem. Cytochem. PubMed Scopus Google Scholar). These application of in the of of a of in the of during the of fixation S. Heat-induced antigen retrieval: mechanisms and application to histochemistry.Prog. Histochem. Cytochem. 2007; 41: 141-200Crossref PubMed Scopus (125) Google Scholar). for FFPE tissue samples been in proteomics studies, to the that tissue fixation proteomic analysis work to this B.L. Conrads T.P. 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These provide that of archival FFPE specimens for retrospective is these we that the and of archival FFPE specimens and with to work the work on shotgun proteomics with FFPE tissue specimens and the of FFPE samples with corresponding fresh frozen is a critical in in retrospective biomarker discovery in the for with
Sprung et al. (Mon,) studied this question.
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